mscv ires ngfr (Addgene inc)
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Mscv Ires Ngfr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mscv+ires+ngfr/NGFR+(Plasmid+%2327489)/bio_rxiv__64898__2026__02__02__701413-203-11-12
Average 93 stars, based on 15 article reviews
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Plasmid Preparation:Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2. Article Snippet: .. Article Title: Novel Clinical Insights: Demographic Study of Apical Periodontitis and the Translational Potential of CRAC Channels as Targets of Chronic Inflammatory Disease Article Snippet: .. Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2 Article Snippet: .. Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into other:Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2. Article Snippet: For surface staining, cells isolated from mice or in vitro culture were directly stained with antibodies and/or fixable live/dead dye with 2% fetal bovine serum (FBS) and 1 mM EDTA at 4°C for 15 min. For transcription factor staining, cells prestained with surface markers were fixed and permeabilized in TF Fix/Perm buffer (BD Biosciences) at 4°C for 20 min, washed once with TF Perm/Wash buffer, and stained with target markers in the TF Perm/Wash buffer at 4°C for 15 min. For intracellular cytokine analysis, cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (750 ng/ml; Sigma-Aldrich) at 37°C for 3 hours in the presence of GolgiStop (BD Biosciences) before staining. Polymerase Chain Reaction:Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into Clone Assay:Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into Retroviral:Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system Article Snippet: .. Schematic representation of the retroviral vectors described in this protocol Top: |

Valle-Noguera et al., 2020 ). Note that IL-10 staining on ex vivo (top) and in vivo (bottom) samples was performed with clone JES5-16E3 (Alexa Fluor 488) and JES5-16E3 (Alexa Fluor 700), respectively. " width="100%" height="100%">