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mscv ires ngfr  (Addgene inc)


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    Structured Review

    Addgene inc mscv ires ngfr
    Mscv Ires Ngfr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mscv+ires+ngfr/NGFR+(Plasmid+%2327489)/bio_rxiv__64898__2026__02__02__701413-203-11-12
    Average 93 stars, based on 15 article reviews
    mscv ires ngfr - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2.
    Article Snippet: .. MSCV-IRES-NGFR (plasmid #27489), MSCV-PIG-Myb (plasmid #66988), and retro–guide RNA (gRNA)–eGFP (plasmid #116926) were purchased from Addgene. .. For surface staining, cells isolated from mice or in vitro culture were directly stained with antibodies and/or fixable live/dead dye with 2% fetal bovine serum (FBS) and 1 mM EDTA at 4°C for 15 min. For transcription factor staining, cells prestained with surface markers were fixed and permeabilized in TF Fix/Perm buffer (BD Biosciences) at 4°C for 20 min, washed once with TF Perm/Wash buffer, and stained with target markers in the TF Perm/Wash buffer at 4°C for 15 min. For intracellular cytokine analysis, cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (750 ng/ml; Sigma-Aldrich) at 37°C for 3 hours in the presence of GolgiStop (BD Biosciences) before staining.

    Article Title: Novel Clinical Insights: Demographic Study of Apical Periodontitis and the Translational Potential of CRAC Channels as Targets of Chronic Inflammatory Disease
    Article Snippet: .. MSCV-IRES-NGFR was from Addgene (plasmid #27489) and kindly provided by Zouming Sun (City of Hope, National Medical Center). ..

    Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2
    Article Snippet: .. MSCV-IRES-NGFR (plasmid #27489), MSCV-PIG-Myb (plasmid #66988), and retro–guide RNA (gRNA)–eGFP (plasmid #116926) were purchased from Addgene. .. For surface staining, cells isolated from mice or in vitro culture were directly stained with antibodies and/or fixable live/dead dye with 2% fetal bovine serum (FBS) and 1 mM EDTA at 4°C for 15 min. For transcription factor staining, cells prestained with surface markers were fixed and permeabilized in TF Fix/Perm buffer (BD Biosciences) at 4°C for 20 min, washed once with TF Perm/Wash buffer, and stained with target markers in the TF Perm/Wash buffer at 4°C for 15 min. For intracellular cytokine analysis, cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (750 ng/ml; Sigma-Aldrich) at 37°C for 3 hours in the presence of GolgiStop (BD Biosciences) before staining.

    Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors
    Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene Plasmid #27489). ..

    Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation
    Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene plasmid 27489). .. Individually, DNA fragments encoding Nr4a1 (a gift from C.-W. J. Lio, La Jolla Institute for Immunology), Nr4a2 (Addgene Plasmid 3500), and Nr4a3 (DNASU plasmid MmCD00080978) were PCR-amplified with 5′ HA-tag and cloned into MSCV-IRES-Thy1.1.

    other:

    Article Title: Steroid nuclear receptor coactivator 2 controls immune tolerance by promoting induced T reg differentiation via up-regulating Nr4a2.
    Article Snippet: For surface staining, cells isolated from mice or in vitro culture were directly stained with antibodies and/or fixable live/dead dye with 2% fetal bovine serum (FBS) and 1 mM EDTA at 4°C for 15 min. For transcription factor staining, cells prestained with surface markers were fixed and permeabilized in TF Fix/Perm buffer (BD Biosciences) at 4°C for 20 min, washed once with TF Perm/Wash buffer, and stained with target markers in the TF Perm/Wash buffer at 4°C for 15 min. For intracellular cytokine analysis, cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (750 ng/ml; Sigma-Aldrich) at 37°C for 3 hours in the presence of GolgiStop (BD Biosciences) before staining.

    Polymerase Chain Reaction:

    Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors
    Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene Plasmid #27489). ..

    Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation
    Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene plasmid 27489). .. Individually, DNA fragments encoding Nr4a1 (a gift from C.-W. J. Lio, La Jolla Institute for Immunology), Nr4a2 (Addgene Plasmid 3500), and Nr4a3 (DNASU plasmid MmCD00080978) were PCR-amplified with 5′ HA-tag and cloned into MSCV-IRES-Thy1.1.

    Clone Assay:

    Article Title: Nr4a transcription factors limit CAR T cell function in solid tumors
    Article Snippet: DNA fragment encoding huCD19 was PCR-amplified and cloned into an MSCV-puro (Clontech) murine retroviral vector. .. DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene Plasmid #27489). ..

    Article Title: Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradation
    Article Snippet: .. Briefly, a DNA fragment encoding Cre was PCR-amplified and cloned into MSCV-IRES-NGFR (Addgene plasmid 27489). .. Individually, DNA fragments encoding Nr4a1 (a gift from C.-W. J. Lio, La Jolla Institute for Immunology), Nr4a2 (Addgene Plasmid 3500), and Nr4a3 (DNASU plasmid MmCD00080978) were PCR-amplified with 5′ HA-tag and cloned into MSCV-IRES-Thy1.1.

    Retroviral:

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system
    Article Snippet: .. Schematic representation of the retroviral vectors described in this protocol Top: MSCV-IRES-NGFR (NGFR, Addgene #27489). .. Bottom: MSCV-IRES-Thy1.1 (Thy1.1, Addgene #17442).



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    Addgene inc mscv ires ngfr ngfr
    Schematic representation of the retroviral vectors described in this protocol Top: <t>MSCV-IRES-NGFR</t> (NGFR, Addgene #27489). Bottom: MSCV-IRES-Thy1.1 (Thy1.1, Addgene #17442).
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    Image Search Results


    Schematic representation of the retroviral vectors described in this protocol Top: MSCV-IRES-NGFR (NGFR, Addgene #27489). Bottom: MSCV-IRES-Thy1.1 (Thy1.1, Addgene #17442).

    Journal: STAR Protocols

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system

    doi: 10.1016/j.xpro.2022.101543

    Figure Lengend Snippet: Schematic representation of the retroviral vectors described in this protocol Top: MSCV-IRES-NGFR (NGFR, Addgene #27489). Bottom: MSCV-IRES-Thy1.1 (Thy1.1, Addgene #17442).

    Article Snippet: MSCV-IRES-NGFR (NGFR) , Addgene , RRID: Addgene_27489.

    Techniques: Retroviral

    Schematic overview of the murine splenic T-cell retroviral transduction and co-culture protocol Step 1: Production of Thy1.1 and NGFR-expression construct-carrying retroviruses in the Plat-E packaging cell line. Step 2: Isolation, activation, and transduction of splenic naïve CD4 + T cells from RORγt WT and RORγt S182A mice. Step 3: Co-culture, polarization, and harvest of retrovirally transduced RORγt WT and RORγt S182A CD4 + T cells marked by cell surface Thy1.1 or NGFR.

    Journal: STAR Protocols

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system

    doi: 10.1016/j.xpro.2022.101543

    Figure Lengend Snippet: Schematic overview of the murine splenic T-cell retroviral transduction and co-culture protocol Step 1: Production of Thy1.1 and NGFR-expression construct-carrying retroviruses in the Plat-E packaging cell line. Step 2: Isolation, activation, and transduction of splenic naïve CD4 + T cells from RORγt WT and RORγt S182A mice. Step 3: Co-culture, polarization, and harvest of retrovirally transduced RORγt WT and RORγt S182A CD4 + T cells marked by cell surface Thy1.1 or NGFR.

    Article Snippet: MSCV-IRES-NGFR (NGFR) , Addgene , RRID: Addgene_27489.

    Techniques: Retroviral, Transduction, Co-Culture Assay, Expressing, Construct, Isolation, Activation Assay

    Comparison of ex vivo co-culture and in vivo intestinal lamina propria T lymphocyte populations Top: Representative flow cytometry plots of transduced RORγt WT (NGFR + ) and RORγt S182A (Thy1.1 + ) CD4 + T cells co-cultured and polarized toward multiple T cell subsets, including Th17 (RORγt + Foxp3 - , blue), RORγt + Treg (RORγt + Foxp3 + , red) and cTreg (RORγt - Foxp3 + , green). Bottom: Representative flow cytometry plots from the small intestinal and colonic lamina propria (siLP and cLP), respectively. Lamina propria T lymphocyte populations were isolated as previously described by ( ; <xref ref-type=Valle-Noguera et al., 2020 ). Note that IL-10 staining on ex vivo (top) and in vivo (bottom) samples was performed with clone JES5-16E3 (Alexa Fluor 488) and JES5-16E3 (Alexa Fluor 700), respectively. " width="100%" height="100%">

    Journal: STAR Protocols

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system

    doi: 10.1016/j.xpro.2022.101543

    Figure Lengend Snippet: Comparison of ex vivo co-culture and in vivo intestinal lamina propria T lymphocyte populations Top: Representative flow cytometry plots of transduced RORγt WT (NGFR + ) and RORγt S182A (Thy1.1 + ) CD4 + T cells co-cultured and polarized toward multiple T cell subsets, including Th17 (RORγt + Foxp3 - , blue), RORγt + Treg (RORγt + Foxp3 + , red) and cTreg (RORγt - Foxp3 + , green). Bottom: Representative flow cytometry plots from the small intestinal and colonic lamina propria (siLP and cLP), respectively. Lamina propria T lymphocyte populations were isolated as previously described by ( ; Valle-Noguera et al., 2020 ). Note that IL-10 staining on ex vivo (top) and in vivo (bottom) samples was performed with clone JES5-16E3 (Alexa Fluor 488) and JES5-16E3 (Alexa Fluor 700), respectively.

    Article Snippet: MSCV-IRES-NGFR (NGFR) , Addgene , RRID: Addgene_27489.

    Techniques: Comparison, Ex Vivo, Co-Culture Assay, In Vivo, Flow Cytometry, Cell Culture, Isolation, Staining

    Expected transduction efficiency in cultured murine CD4 + T cells Top: Flow cytometry gating strategy for evaluating transduction efficiency in live CD4 + T cells. Bottom: Proportions of live NGFR + or Thy1.1 + CD4 + T cells 72 h post-polarization. Data are represented as mean ± SD.

    Journal: STAR Protocols

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system

    doi: 10.1016/j.xpro.2022.101543

    Figure Lengend Snippet: Expected transduction efficiency in cultured murine CD4 + T cells Top: Flow cytometry gating strategy for evaluating transduction efficiency in live CD4 + T cells. Bottom: Proportions of live NGFR + or Thy1.1 + CD4 + T cells 72 h post-polarization. Data are represented as mean ± SD.

    Article Snippet: MSCV-IRES-NGFR (NGFR) , Addgene , RRID: Addgene_27489.

    Techniques: Transduction, Cell Culture, Flow Cytometry

    Journal: STAR Protocols

    Article Title: Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system

    doi: 10.1016/j.xpro.2022.101543

    Figure Lengend Snippet:

    Article Snippet: MSCV-IRES-NGFR (NGFR) , Addgene , RRID: Addgene_27489.

    Techniques: Recombinant, Modification, Infection, Transfection, Staining, Plasmid Preparation, Cell Isolation, Bacteria, Retroviral, Functional Assay, Software